气味 Odor: characteristic

采用 Use: 当归素是从补骨脂中分离得到的天然呋喃香豆素。水果 Angelicin is a natural furocoumarin isolated from Psoralea corylifolia L. fruit,

气味 Odor: characteristic

采用 Use: 当归显示出各种各样的有效和有趣的生物活性。这些包括抗真菌活动 Angelicin displays a wide variety of potent and interesting biological activities. These include antifungal activities, 在rad 14突变体中引起s相延迟 inducing S-phase delay in the rad 14?mutant, 抑制2-氨基-3-甲基咪唑的诱变[4 inhibition of mutagenesis of 2-amino-3-methylimidazo[4,5-f]喹(+)-香柏酮 5-f]quinoloine, 黄曲霉毒素b1向黄曲霉毒素b1-8生物转化的抑制作用 inhibitory effects on the biotransformation of aflatoxin B1 to aflatoxin B1-8,9-环氧化物 9-epoxide, 和抑制诱导型一氧化氮合酶。 and inhibition of inducible nitric oxide synthase. 当归霉素与补骨脂素在结构上相关 Angelicin is structurally related to psoralens, 光敏剂的一种众所周知的化学类别，因其具有抗增殖活性，可用于治疗各种皮肤疾病。验证当归的活性 a well-known chemical class of photosensitizers used for its antiproliferative activity in treatment of different skin diseases. To verify the activity of angelicin, 我们使用了 类sh-sy5y橙花母细胞瘤细胞来研究其细胞毒性 we employed human SH-SY5Y neuroblastoma cells to investigate its cytotoxicity, 尽管其作用机理尚未完全阐明。这里 although its mechanism of action has not yet been fully elucidated. Here, 我们通过细胞生存力分析检查了当归霉素的细胞毒性 we examined the cellular cytotoxicity of angelicin by cell viability assay, 通过dna阶梯分析进行dna片段化 DNA fragmentation by DNA ladder assay, 和原子量[14.00643; 14.00728]。氮以双原子气体的形式存在，占地球大气总量的78％。它是蛋白质和核酸组成部分，存在于所有活细胞中。在食品中用作推进剂和充气剂 and activation of caspases and Bcl-2 family proteins by western blot analyses. The results of our investigation suggest that angelicin increased cellular cytotoxicity in a dose- and time-dependent manner with IC(50) of 49.56 �M at 48 h of incubation. In addition, 当归霉素剂量依赖性下调包括bcl-2在内的抗凋亡蛋白的表达 angelicin dose-dependently downregulated the expression of anti-apoptotic proteins including Bcl-2, bcl-xl Bcl-xL, 和mcl-1提示不参与fas / fasl诱导的caspase-8介导的外源性内在的线粒体介导的凋亡途径的参与 and Mcl-1 suggesting the involvement of the intrinsic mitochondria-mediated apoptotic pathway which did not participate in Fas/FasL-induced caspase-8-mediated extrinsic, 定位激酶 MAP kinases, 和pi3k / akt / gsk-3＆beta;途径。此外 and PI3K/AKT/GSK-3β pathway. Furthermore, 我们阐明了caspase-9和caspase-3的剂量依赖性上调，这表明当归霉素诱导的凋亡主要通过内在的caspase介导的途径介导。特别是 we clarified the dose-dependent upregulation of caspase-9 and caspase-3 which indicated that angelicin-induced apoptosis is mediated primarily through the intrinsic caspase-mediated pathway. In particular, caspase-3抑制剂 the caspase-3 inhibitor, devd-fmk DEVD-fmk, 诱导了当归霉素诱导的细胞毒性的降低，这证实了在凋亡过程中固有的caspase依赖性途径并未使用map激酶和gsk-3抑制剂阻止细胞毒性。在一起 induced a reduction in angelicin-induced cytotoxicity which confirmed that the intrinsic caspase-dependent pathway during this apoptosis which did not prevent cytotoxicity using MAP kinases and GSK-3 inhibitor. Taken together, 我们的数据表明当归素是 类sh-sy5y橙花母细胞瘤细胞的有效诱导凋亡的天然化合物，这表明该化合物可能在 类橙花母细胞瘤癌症的未来治疗中发挥作用。 our data shows that angelicin is an effective apoptosis-inducing natural compound of human SH-SY5Y neuroblastoma cells which suggests that this compound may have a role in future therapies for human neuroblastoma cancer.